Recombinant DNA, transformant containing said DNA, and process for preparing heat-stable glucose dehydrogenase by use of said transformant
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United States of America Patent
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May 19, 1992
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N/A
app pub date -
Sep 22, 1989
filing date -
Sep 22, 1988
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Expired
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Abstract
A recombinant DNA replicable and expressible in Escherichia coli is disclosed which encodes glucose dehydrogenase originating from Bacillus megaterium. A DNA sequence encoding the glucose dehydrogenase is incorporated into an E. coli vector and then used to transform an E. coli host. Also disclosed are transformants and recombinant DNA incorporating thermostable glucose dehydrogenase, wherein at least one amino acid in glucose dehydrogenase at positions 96, 252, and 253 is replaced by a thermostability-conferring amino acid.
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