Composition and method for isolation and purification of Immunoglobulin M
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United States of America Patent
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May 12, 1992
Grant Date -
N/A
app pub date -
Sep 28, 1990
filing date -
Sep 28, 1990
priority date (Note) -
Expired
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Abstract
Preparation of a composite of mannan binding protein attached to an insoluble, support matrix is accomplished by reacting cyanogen bromide activated beaded agarose with a buffered solution of mannan binding protein isolated from rabbit serum. The composite has utility as an affinity sorbent for IgM when divalent metal ions are incorporated in the binding buffer. The composite does not show cross-reactivity (binding) with immunoglobulins of the G class.
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