Factor VIII complex purification using heparin affinity chromatography
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United States of America Patent
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May 5, 1992
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N/A
app pub date -
Aug 1, 1989
filing date -
Aug 1, 1989
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Expired
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Abstract
There is provided in accordance with the practice of this invention, a process for separating Factor VIII complex from an impure protein fraction containing Factor VIII complex. An aqueous solution of the impure protein fraction containing Factor VIII complex is applied to a heparin coupled chromatographic medium to bind the Factor VIII complex to the medium. The Factor VIII is then recovered from the heparin coupled chromatographic medium by elution with an aqueous CaCl.sub.2 solution.
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