Method of gene mapping
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United States of America Patent
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Apr 7, 1992
Issued Date -
N/A
app pub date -
Apr 25, 1988
filing date -
Sep 28, 1987
priority date (Note) -
Expired
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Abstract
The method described characterizes each DNA segment to be mapped by cleaving it to produce DNA fragments which are then end labeled with a reporter(s) specific to the end nucleotides of each fragment. The labeled fragments are again cleaved to produce short fragments which are separated according to size. The short fragments are analyzed as to report identify and size which is indicative of the character of each fragment. By derivatizing the cleaved ends of the primary cleaved fragments, the labeling may be delayed until the second cleavage. Prior to the labeling the derivatized fragments, all underivatized fragments are removed, the derivatized fragments being immobilized.
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