Nucleic acid separation method
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United States of America Patent
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Mar 17, 1992
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N/A
app pub date -
Jun 4, 1990
filing date -
Jun 4, 1990
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Expired
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Abstract
An improved method for isolating and purifying nucleic acid from cell culture media contemplating adding to resuspended cell solutions a lysing solution and neutralizing/deproteinating agent without mechanical mixing, followed by centrifugation to partially pellet cellular debris, mixing the solution to complete the reaction, and additional centrifugation to fully pellet the debris.
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