A pickup mechanism includes a main frame, a supporting element mounted to the main frame, and a pickup module positioned in the main frame and including a pickup arm, a transmitting element, a separation element, a pickup element and a barrel element. The separation element includes a separation shaft and a separation roller. The pickup element includes a pickup shaft and a pickup roller. The separation roller and the pickup roller are restricted in the pickup arm. The transmitting element links the separation shaft and the pickup shaft. The pickup arm is pivoted at the separation shaft. The barrel element has a base portion pivotally mounted around the pickup shaft, and a stopping block. When the pickup module swings upward or downward, the barrel element is driven to rotate along with the pickup shaft to make the stopping block against the supporting element or apart from the supporting element.

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