Method for measurement of .alpha.-amylase activity
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United States of America Patent
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Nov 26, 1991
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N/A
app pub date -
Jan 3, 1991
filing date -
Nov 20, 1986
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Abstract
A for measurement of .alpha.-amylase activity uses a substrate comprising a malto-oligo saccharide represented by general formula (I) or (V) described below: A - Gn - B (I) A - Gn - I (V) wherein A represents: ##STR1## B represents a monosaccharide or a derivative thereof other than glucose; I represents inositol or a derivative thereof; G represents glucose; and n represents an integer of 3 to 15; in formula (II) or (III), R.sub.1 to R.sub.4 each represents a hydrogen atom, a lower alkyl group or (CH.sub.2)yCOOM (wherein y is 0, 1 or 2 and M represents a hydrogen atom or an alkali metal); and X.sub.1 to X.sub.4 each represents an oxygen atom or a sulfur atom. The method for measurement of .alpha.-amylase activity comprises contacting a substrate containing a malto-oligo saccharide represented by general formula (I) or (V) described above with a sample in the presence of glucosidase and measuring a liberated monosaccharide, inositol or a derivative thereof thereby to measure .alpha.-amylase activity in the sample.
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