Method for separating nucleic acids and nucleic acid probes
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United States of America Patent
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Oct 22, 1991
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N/A
app pub date -
Nov 16, 1987
filing date -
Dec 26, 1985
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Expired
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Abstract
An improved method and device for nucleic acid hybridization assay employing combined direct an alternating field electrophoresis are disclosed. In the method of the present invention, a sample is hybridized with nucleic acid probe and is contacted with a support medium where direct and alternating electric fields are applied. Under the influence of the electric fields, hybrid separates from non-specifically bound nucleic acid probe. The hybrid may be measured on the support medium itself as on a paper strip or in a cartridge containing support medium or may be blotted on an inert surface and then measured. The method an device are useful in the diagnosis of diseases. Kits are provided for assay of a large number of diseases.
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