Method for the measurement of creatine or creatinine and reagents for these measurements
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Sep 10, 1991
Grant Date -
N/A
app pub date -
Jan 5, 1988
filing date -
Jan 23, 1987
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A method for measuring creatine in a sample by the use of creatine amidinohydrolase which comprises decomposing the N-ethylglycine present in the sample enzymatically and thereafter reacting sarcosine oxidase upon the sample; and a reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 20 mM or below and catalase or comprises said sarcosine oxidase, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, a sarcosin oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 50 mM or above, peroxidase and a color reagent for H.sub.2 O.sub.2.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully