Process for the long-term surviving culture of hepatocytes
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United States of America Patent
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Jul 9, 1991
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app pub date -
Nov 15, 1989
filing date -
Jun 6, 1985
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Abstract
A process is disclosed for the long term surviving culture of hepatocytes maintaining their morphological and functional characteristics for long-term-periods, suitable to assest the acute and chronic hepatotoxicity of drugs, chemicals and environmental pollutants. The long term surviving culture of hepatocytes is carried out in a culture media containing fibroblast cells or fibroblast cell products treated to prevent cell multiplication and inoculated under controlled density.
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