Scattered total internal reflectance immunoassay system
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United States of America Patent
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May 21, 1991
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N/A
app pub date -
Jan 27, 1988
filing date -
Jun 26, 1986
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Expired
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Abstract
A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio.
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