DNA vectors and their use in recombinant DNA technology
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United States of America Patent
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May 14, 1991
Issued Date -
N/A
app pub date -
Dec 5, 1988
filing date -
Mar 25, 1983
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Expired
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Abstract
A new class of DNA vectors, each comprising two replication systems; a first origin of replication resulting in a low copy number and stable inheritance of the plasmid, and a second, high copy number, origin of replication at which replication is directly controllable such that, when host cells carrying the vector are propagated under a first set of conditions, replication takes mainly from the low copy number origin, and that when said cells are propagated under a second set of conditions, replication takes place also from the high copy number origin to produce a high yield of gene product. The controllable origin of replication may be under the control of a natural promoter of RNA transcription or a substitute promoter such as the PL promoter or lac promoter.
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