Recombinant DNA-molecules and method for protein production
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United States of America Patent
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Apr 23, 1991
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N/A
app pub date -
Nov 30, 1987
filing date -
Dec 31, 1980
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Abstract
The invention relates to recombinant DNA molecules and to methods for producing proteins by means of said molecules. Particularly, the present invention relates to recombinant DNA molecules which are synthetized in Bacillus strain bacteria and are characterized by DNA which codes for exoenzymes excreted by a bacterium of the Bacillus strain and which are present in tens of copies in Bacillus strain bacteria, as well as to recombinant DNA molecules which are modified from the above recombinant DNA molecules and are characterized by DNA containing the regulation and excretion signals of the .alpha.-amylase gene of B. amyloliquefaciens, to which signals a gene of any protein can be joined. These recombinant DNA molecules can be used, for example, to improve the production of .alpha.-amylase in Bacillus strain bacteria, and their modifications to produce any protein in Bacillus strain bacteria.
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