Method for the preparation of a selected protein or a part thereof in Bacillus strain bacteria
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United States of America Patent
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Apr 23, 1991
Issued Date -
N/A
app pub date -
Nov 30, 1987
filing date -
Dec 31, 1980
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Abstract
A selected protein or protein part is prepared in Bacillus strain bacteria by joining DNA encoding the selected protein or protein part essential to its biological activity to a bacterium gene. The Bacillus amyloliquefaciens gene for .alpha.-amylase is cleaved at a location after the excretion signal following the regulation sequence, or at a location after a part essential with respect to its excretion. The cleaved gene is joined to a plasmid present in Bacillus strain bacteria in several copies, and the DNA sequence encoding the selected protein or protein part essential to its biological activity is joined to the cleavage site by recombinant DNA techniques. Bacillus strain host bacteria are transformed with the recombinant DNA molecules so obtained, and the transformed host bacteria cultivated to produce the selected protein or protein part.
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