Extrusion technique for producing unilamellar vesicles
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United States of America Patent
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Apr 16, 1991
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N/A
app pub date -
Feb 13, 1989
filing date -
Jun 20, 1984
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Expired
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Abstract
A method for reducing the lamellarity of a population of liposomes is provided which comprises repeatedly passing the liposomes under pressure through a filter which has a pore size equal to or less than about 100 nm. In certain embodiments, the method is used to convert a population of previously formed multilamellar liposomes into a population of substantially unilamellar liposomes. In accordance with other aspects of the disclosure, liposomes are prepared directly from a lipid powder or pellet and buffer without the use of any solvents, detergents or other extraneous materials.
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