Method of preparing membrane filters for transmission electron microscopy
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United States of America Patent
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Apr 2, 1991
Issued Date -
N/A
app pub date -
Jun 12, 1990
filing date -
May 31, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
This application relates to a method for preparing specimens for viewing particles and/or fibers captured upon membrane filters with a TEM. The method comprises mounting a section of the membrane filter on a glass slide with a quick drying liquid applied between the filter membrane and the slide which liquid will not release the filter membrane when the slide is immersed in a selected solvent and such that the liquid will not render the filter membrane resistant to the selected solvent. Next, the filter section surface is coated with carbon to replicate the surface of the membrane filter and the particles and fibers thereupon. The coated membrane is then immersed in the selected solvent which will attack the filter material causing the carbon film squares to float off of the memberane filter.
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