Lipase, its production and use for assay of triglycerides

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United States of America Patent

PATENT NO 4999289
SERIAL NO

07034452

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Abstract

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Lipase isolated from Penicillium characterized by the following properties: (1) Efficiently hydrolyzes triglycerides of fatty acids having 4-18 carbon atoms; (2) Hydrolysis of such triglycerides with such lipase produces at least 5 moles of glycerol per 100 moles of fatty acid; (3) Optimal pH range for said hydrolysis 5-7; (4) Stable in the pH range of 4.5-6; (5) Optimal temperature range for activity 35.degree.-40.degree. C.; (6) thermal stability up to about 35.degree. C.; (7) Activated by surface-active agents, but not substantially inhibited by such surface-active agents in concentration up to 5%; (8) Molecular weight about 1000,000 to 120,000; (9) Isoelectric point about pH 3.84.

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Patent Owner(s)

Patent OwnerAddress
AMANO PHARMACEUTICAL CO LTD A CORP OF JAPAN2-7 NISHIKI 1-CHOME NAKA-KU NAGOYA-SHI AICHI-KEN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Akiba, Tetsunori Kani, JP 2 20
Isobe, Kimiyasu Konan, JP 11 21
Matsunaga, Kuniyoshi Ichinomiya, JP 8 53
Suzuki, Satoru Aichi, JP 137 1284
Yamaguchi, Shotaro Aichi, JP 63 284

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