Method and structures employing chemically-labelled polynucleotide probes
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United States of America Patent
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Feb 19, 1991
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app pub date -
Jul 20, 1989
filing date -
Jan 27, 1983
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Abstract
Polynucleotide sequences in a sample of biological or nonbiological material are detected by a method involving fixing of the sequences on a solid support and forming an entity between the fixed sequences and chemically-labeled polynucleotide or oligonucleotide probes having a sequence complementary to the fixed sequence for determining the identification and/or presence of the target polynucleotide sequences. The chemical label covalently or noncovalently attached to the probe comprises a signalling moiety capable of generating a soluble signal detectable by spectrophotometric assay techniques.
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