Enzymes immobilized on latex polymer particles for use with an amino acid electrosensor
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Jan 8, 1991
Grant Date -
N/A
app pub date -
Oct 26, 1988
filing date -
Oct 29, 1987
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
Enzymes such as L-methionine gamma lyase, L-lysine decarboxylase, L-aspartase and L-tryptophanase are immobilized by contacting with a latex of polymer particles having a negative surface charge such that the electrophoretic mobility of the latex has a negative value of -2.0 10.sup.-8 m.sup.2 V.sup.-1 sec.sup.-1 or below when measured at pH 7 in 0.01M KNO.sub.3 and a polymer solids concentration of 30 mg/l. The polymer particles also have a hydrophobic surface such that the contact angle of a 1 microliter droplet of distilled water with a plane horizontal surface formed by drying and compacting the latex particles is 70.degree. or more. The resultant-enzyme/polymer particles can be used for assaying amino acids corresponding to the specific enzyme immobilized in aqueous solutions using an electrosensor comprising a pH-sensitive electrode located in a sensing zone or chamber containing the enzyme/polymer particles as an aqueous dispersion or in water-dispersible form.
First Claim
Family
International Classification(s)
- [Classification Symbol]
- [Patents Count]
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully