Cuvette for receiving liquid sample
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United States of America Patent
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Dec 25, 1990
Issued Date -
N/A
app pub date -
Aug 1, 1989
filing date -
Jan 27, 1988
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.
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