Improved method for assaying proteolytic enzymes
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United States of America Patent
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Aug 21, 1990
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N/A
app pub date -
Aug 15, 1986
filing date -
Aug 15, 1986
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Abstract
A reagent for proteolytic enzyme assays has the general formula ##STR1## where RCO-- is an enzyme reactive acyl, such as an amino acid, peptide or substituted amino acid or peptide. The reagent may be hydrolysed by proteolytic enzymes and developed to form a distinctive color. The reagent may be formed by reacting RCOOH with N-hydroxysuccinimide to form the acyl N-hydroxysuccinimide ester. The ester may then be reacted to form the reagent.
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