Electrophoresis imaging system
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United States of America Patent
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Jun 5, 1990
Grant Date -
N/A
app pub date -
Mar 25, 1988
filing date -
Mar 25, 1988
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Expired
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Abstract
A method and system for imaging unstained smaple material constituents on a gel electrophoresis unit. The system includes a gel electrophoresis unit, preferably having a pH gradient gel thereon, phase detection optics including a collimated light source, shearing interferometer, phase contrast microscope or schlieren microscope, an image digitizer, and data processing electronics, such as a computer, for determining the concentration of sample constituents at each position on the electrophoresis unit. The method relies on the difference in index of refraction between sample material and gel material to introduce optical path differences in light rays passing through the material. The modified light is subjected to shearing interferometry to locate gel-sample boundaries, phase imaging to directly image the sample, or a laser scanner for directly measuring the deviation in a light beam due to the sample.
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