Eucaryotic expression vectors
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United States of America Patent
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May 15, 1990
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N/A
app pub date -
Mar 18, 1986
filing date -
Jul 20, 1984
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Expired
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Abstract
A eukaryotic expression vector includes a controllable repressor operator sequence witin the expression control sequence of the vector. The controllable repressor operator sequence represses expression of a heterologous structural gene located downstream of the expression control sequence in the presence of a gene product or a combination of gene products of a yeast mating type locus. This allows control of the expression level of the heterologous structural gene. Controllable repressor operator sequences responsive to the a1/.alpha.2 and .alpha.2 gene products of the MATa and MAT.alpha. yeast mating type locus alleles are described, both derived from genetic material and synthesized chemically.
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