DNA sequence
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United States of America Patent
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Feb 6, 1990
Issued Date -
N/A
app pub date -
Feb 29, 1988
filing date -
May 31, 1983
priority date (Note) -
Expired
status (Latency Note)
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Abstract
An upstream activator sequence derived from the yeast PGK gene. The upstream activator sequence is contained in the 5' region of the yeast PGK gene between nucleotides -324 and -455. The upstream activator sequence has synthetic linkers attached to either end to facilitate attachment to a vector. Vectors including the upstream activator sequence and a heterologous promoter sequence are described. The upstream activator sequence is used in a method for increasing the expression level of a yeast expression vector.
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