Method for the determination of cholesterol
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United States of America Patent
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Jan 9, 1990
Issued Date -
N/A
app pub date -
Dec 22, 1987
filing date -
Oct 31, 1984
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Abstract
An enzymatic method for measurement of cholesterol comprises incubating: (a) a test sample; (b) a cholesterol dehydrogenase; (c) an oxidizing agent selected from the group consisting of nicotinamide -adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and (d) a surfactant and measuring the resulting detectable oxidized and reduced products kinetically. A composition for the kinetic measurement of cholesterol comprises: (a) a cholesterol dehydrogenase; (b) an oxidizing agent selected from the group consisting of nicotinamide -adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and (c) a surfactant.
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