Detection and differentiation of coxiella burnetii in biological fluids
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United States of America Patent
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Oct 24, 1989
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app pub date -
Nov 5, 1985
filing date -
Nov 5, 1985
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Expired
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Abstract
Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.
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