Novel bacterium inducible to produce unique steroid-transforming enzymes: preparation and applications thereof
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United States of America Patent
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Oct 17, 1989
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app pub date -
Oct 23, 1985
filing date -
Oct 23, 1985
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Abstract
The present invention discloses a unique biologically pure culture of a microorganism belonging to Alcaligenes species. The microorganism has the property of producing novel 17.beta.-hydroxysteroid dehydrogenase (17.beta.-HSD) capable of enzymatic reaction with an androgen and an estrogen. The methods of isolation the microorganism and the 17.beta.-HSD have been described. An assay and a kit for detecting the presence of C.sub.18 and C.sub.19 steroids in biological samples have also been disclosed.
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