Simultaneous enzyme immunoassay for detecting antigen and/or antibody in humans
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United States of America Patent
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Sep 26, 1989
Grant Date -
N/A
app pub date -
Jun 15, 1987
filing date -
Jun 15, 1987
priority date (Note) -
Expired
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Abstract
A solid-phase immunoassay is provided for determination of members of an immunological pair such as antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or 'spike' of the binding pair to the test sample prior to incubation of the test sample in the presence of a solid-phase absorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispensing antigens which may be present in the test sample.
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