Process for solid phase-sequencing of nucleic acid fragments
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United States of America Patent
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Jul 18, 1989
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N/A
app pub date -
Jul 31, 1985
filing date -
Aug 6, 1984
priority date (Note) -
Expired
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Abstract
A process for solid phase sequencing of nucleic acid fragments is disclosed. The object of the invention is to provide a sequencing process which enables the simultaneous sequencing of large amounts of long and short nucleic acid fragments and which is optionally automated. Thus, a solid support combining mechanical stability, anion exchange characteristics, and chemical elution of nucleic acids off the support is used. Immobilized nucleic acid fragments are chemically modified and subsequently the nucleic acid backbone is cleaved and simultaneously or subsequently said fragments are eluted by chemical means. The present invention is applied to molecular biology and gene technology.
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