Method of measuring lipid-bound sialic acid
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Jun 6, 1989
Grant Date -
N/A
app pub date -
Apr 20, 1987
filing date -
Apr 25, 1986
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A method of measuring lipid-bound sialic acid (LSA) which comprises, mixing a LSA separating agent comprising a polar solvent with a sample containing LSA and other sialic acid(s), separating the precipitate formed to obtain a supernatant, and measuring the sialic acid concentration of said supernatant by using an enzyme capable of acting on sialic acid in a lipid-bound state. When a sample is treated with the LSA separating agent comprising a polar solvent, various components in the sample such as protein-bound sialic acid and proteinous substances precipitate while the desired lipid-bound sialic acid remains in the supernatent. Accordingly, by measuring sialic acid concentration of the supernatent, LSA concentration of the sample is easily determined. The method of the invention is simple and superior in reproducibility. The method of the invention is suitable for treating a large number of samples, and it can easily by automated. This method is suitable as a screening test for cancer.
First Claim
Family
International Classification(s)
- [Classification Symbol]
- [Patents Count]
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully