Bacteria for expressing a polysaccharide depolymerase containing a novel recombinant plasmid
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United States of America Patent
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Apr 18, 1989
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N/A
app pub date -
Oct 7, 1985
filing date -
Oct 7, 1985
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Abstract
A segment of a bacteriophage encoding for a polysaccharide depolymerase which has been cloned and expressed in Escherichia coli is described. In particular, bacteriophage ERA103 was found to consist of five EcoRI fragments labeled: A, 7.5-kb; B, 5.0-kb; C, 2.7-kb; D, 2.1-kb and E, 1.8-kb. Fragment B encodes for the depolymerase and was cloned into the positive-selection vector pOP203(A.sub.2.sup.+), pBR322 and the expression vector pKK223-3. The depolymerase is applied to rosaceous plants to prevent Fireblight caused by Erwinia amylovora by depolymerizing a polysaccharide produced by this bacteria.
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