Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
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United States of America Patent
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Apr 11, 1989
Grant Date -
N/A
app pub date -
Nov 23, 1984
filing date -
Nov 23, 1984
priority date (Note) -
Expired
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Abstract
Lesions in genetic sequences, for example, the sickle cell anemia mutation in the beta globin gene, are detected by means of interactive labels in a nucleic acid hybridization assay. A signal is generated only if the labels are in physical proximity which is made dependent on whether a predetermined normal or abnormal sequence is present by exposure to appropriate conditions such as restriction enzyme digestion or stringent hybridization.
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