Chemical blocking agent against non-specific binding or staining of an antibody specific for terminal deoxynucleotidyl transferase in biological specimens during immunoassay
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United States of America Patent
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Apr 4, 1989
Grant Date -
N/A
app pub date -
Sep 15, 1986
filing date -
Sep 15, 1986
priority date (Note) -
Expired
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Abstract
A chemical agent is provided for significantly preventing or blocking non-specific staining or binding of an antibody specific for Terminal deoxynucleotidyl Transferase (TdT) during immunofluorescent or immunoperoxidase assay procedures. These procedures include both immunofluorescent and immunohistochemical staining of samples followed by flow cytometric and/or microscopic analysis, respectively. The invention is practiced by selective use of casein introduced into the assay procedures at an appropriate interval prior to analysis using a labelled or tagged monoclonal antibody specific to a TdT epitope. The casein utilized successfully was obtained from a large variety of sources and includes the use of a non-fat milk product.
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