Processes and materials for carrying out microchemical and microbiological tests
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United States of America Patent
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Jan 17, 1989
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N/A
app pub date -
Feb 20, 1986
filing date -
Jun 22, 1984
priority date (Note) -
Expired
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Abstract
A microbiological culture test process for detecting the presence of nitrate-reducing microorganisms in an inoculum sample, which comprises (a) culturing a microorganism to be tested in the presence of adequately nitrite-free nitrate, for a period sufficient to allow reduction of nitrate by any microorganism (if present) that has the capacity to reduce nitrate to nitrite, (e.g. 1-6 hours); (b) exposing the culture medium after culture to (diazotizing) acid conditions in the presence of an amino-containing fluorophor thereby to cause diazotization of the fluorophor to the extent of any nitrite present in the medium to form a diazonium derivative (or further reaction product thereof) which is substantially less fluorescent (or fluoresces at a substantially different wavelength) than said amino-containing fluorophor, and (c) assessing the fluorescence of the culture medium as treated by step (b), thereby to show a reduction in fluorescence in the presence of a nitrate-reducing microorganism.
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