Process for the biotechnological preparation of poly-d-(-)-3-hydroxybutyric acid
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United States of America Patent
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Nov 22, 1988
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N/A
app pub date -
Nov 23, 1984
filing date -
Dec 1, 1983
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Expired
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Abstract
The present invention relates to a process for the biotechnological preparation of poly-D-(-)-3-hydroxybutyric acid (PHB) which comprises continuously culturing a microorganism that is a strain of Alcaligenes latus or a PHB-producing mutant thereof, in two separate successive fermentation stages with unrestricted supply of nutrients. The PHB-containing microorganism population cultured in the first stage with a dissolved oxygen content of 25 to 50% of the saturation value for air is then continuously transferred into a second fermentation stage in which the culture is continued at a dissolved oxygen content of 8-15% of the saturation value of air. The PHB is extracted in the usual manner from the biomass thereby obtained.
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