Method of determining the nicotine content of tobacco
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United States of America Patent
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Nov 8, 1988
Grant Date -
N/A
app pub date -
Jan 13, 1987
filing date -
Jan 13, 1986
priority date (Note) -
Expired
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Abstract
A method of determining on a discrete analyzer the nicotine content of tobacco or a smoking-related product by preparing an aqueous extract of a sample of tobacco or smoking-related product, pre-reacting the extract with an acidic buffered solution of pH not more than 6 for at least two minutes, reacting the pre-reacted extract in the discrete analyzer with aniline and cyanogen bromide, measuring the intensity of yellow coloration generated, and comparing said intensity with that produced by a nicotine standard to determine the nicotine content of said sample.
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