Monoclonal antibody that recognizes a structure common to human interleukin-2 (TCGF) and to the light lambda chain of human immunoglobulin and lines of hybridoma cells that produce these monoclonal antibodies
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United States of America Patent
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Jun 14, 1988
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N/A
app pub date -
Aug 15, 1984
filing date -
Aug 16, 1983
priority date (Note) -
Expired
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Abstract
Monoclonal antibodies that recognize a structure common to human interleukin-2 and to the light .lambda. chain of human immunoglobulin and lines of hybridoma cells that produce these monoclonal antibodies can be prepared by immunizing animals, especially mice, with human interleukin-2 (TCGF) and fusing the splenocytes obtained from the animals with animal, expecially mouse, myeloma cells to create a hybridoma. The hybridomas are raised as clones and the antibodies obtained from the individual clones tested for their specificity to human interleukin-2 (TCGF). Clones that produce antibodies with a specificity to human interleukin-2 (TCGF) are selected for further raising to prepare the antibody. The antibody is harvested from the culture medium or from the ascitic fluids of the animal, especially the mouse, with the hybridoma.
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