Transfection high voltage controller
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United States of America Patent
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Jun 7, 1988
Grant Date -
N/A
app pub date -
Jun 6, 1986
filing date -
Jun 6, 1986
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A transfection high-voltage controller for directing high-voltage currents to a suspension of cells and DNA comprising a control section, a power store, and a high-joule switch. Under control of the control section, the high-joule switch directs a high-voltage current from the power store to a load such as a cuvette. The high-joule switch comprises a semiconductor controlled rectifier (SCR) having a gate coupled to the control unit for triggering the device, an anode for receiving the high-voltage current from the power store, and a cathode for delivering the high-voltage current to the cuvette. A current sink may be connected to the anode of the SCR to render it non-conductive a predetermined time after the high-voltage current appears at the cathode. Multiple SCRs may be connected in series to support voltages over 3,000 and currents in excess of 125 amperes, and a trigger control unit may be attached to the gate of each SCR in the series to effect simultaneous or serial triggering of the group.
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