Method for forestalling the hook effect in a multi-ligand immunoassay system
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United States of America Patent
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May 10, 1988
Grant Date -
N/A
app pub date -
Apr 11, 1985
filing date -
Apr 11, 1985
priority date (Note) -
Expired
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Abstract
A method is provided for substantially increasing the dynamic range of an immunoassay and forestalling the hook effect observed with very large ligand concentrations in aqueous samples. The method comprises the addition of ligand binding partners which do not have labels associated therewith to compete with labeled ligand binding partners thereby effectively eliminating the presence of the excess ligand in the sample solution. The conversant method for ligand binding partner immunoassays is also provided.
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