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Abstract

The present invention provides a composition for assaying hydrogen peroxide having an enzyme, a buffer and a chromogen, wherein the chromogen is a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 may be the same or different and each represent a straight-chain alkyl group having 1 to 6 carbon atoms, such as a methyl, ethyl, n-propyl, n-butyl, n-amyl or n-hexyl group. R.sub.5 and R.sub.6 each represent a hydrogen atom or a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, with the proviso that either R.sub.5 and R.sub.6 represents a sulfoalkyl group and the sulfoalkyl group may be substituted with at least 1, preferably 1 to 2 hydroxyl groups. This composition has a high solubility in water and is usable over a wide pH range and for the preparation of both a test solution and a test piece. Various modifications of the composition are possible. Therefore, by using the composition, components of a body fluid can be assayed quantitatively in a precise, easy and rapid manner.

First Claim