Methods and materials for obtaining microbial expression of polypeptides including bovine prolactin
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United States of America Patent
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May 19, 1987
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N/A
app pub date -
Dec 1, 1982
filing date -
Dec 1, 1982
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Expired
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Abstract
Disclosed are methods for selective modification of double-stranded DNA sequences facilitating their storage and incorporation into expression vectors. Manufactured partially double-stranded DNA primers are hybridized to complementary regions of single-stranded forms of DNA sequences to be altered. Desired, selectively modified double-stranded DNA sequences are then formed by DNA polymerization and specific nuclease digestion of undesired double- and single-stranded regions. Illustratively provided are DNA sequences useful in the microbial expression of bovine prolactin and other valuable polypeptides such as avian growth hormone.
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