Peptide derivatives and use thereof as substrates for quantitatively assaying enzymes
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United States of America Patent
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Dec 16, 1986
Grant Date -
N/A
app pub date -
Jun 1, 1984
filing date -
Jun 3, 1983
priority date (Note) -
Expired
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Abstract
Peptide derivatives of formula ##STR1## wherein R.sup.1 represents a substituted amino group which is capable of being split off enzymatically with formation of a colored or fluorescent compound R.sup.1 -H. The said peptide derivatives are used for quantitatively assaying the enzyme C.sub.1 -esterase. The assaying is carried out by reacting a medium which contains C.sub.1 -esterase or in which the latter is formed or consumed with a peptide derivative as defined above and measuring photometrically, spectrophotometrically, fluorescence-spectrophotometrically or electrochemically the quantity of split product R.sup.1 -H released per time unit by the catalytic hydrolytic action of the said enzyme on the peptide derivative.
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