Reagents containing an anti-ligand bound to an anti-enzyme and methods for employing said reagents in an immunoassy
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United States of America Patent
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Nov 4, 1986
Grant Date -
N/A
app pub date -
Mar 12, 1984
filing date -
Mar 11, 1983
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The invention relates to a method and novel reagents for detecting a ligand in a biological sample. In the method, the sample is combined with (i) an enzyme or an enzyme bound to a first macromolecular compound, (ii) substrate for the enzyme, and (iii) an antibody complex comprising anti-ligand bound to anti-enzyme and a second macromolecular compound bound to either one or both of the anti-ligand and anti-enzyme. The activity of the enzyme varies as a function of the unknown amount of ligand contained in the biological sample.
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