Reagent for measuring direct bilirubin by enzymatic method and method for measurement thereof
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United States of America Patent
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Feb 18, 1986
Grant Date -
N/A
app pub date -
Dec 19, 1983
filing date -
Dec 29, 1982
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Reagent for the measurement of direct bilirubin comprising a buffer solution having a pH range of 3.5 to 4.5 which contains bilirubin oxidase, preferably in the form of a test kit consisting essentially of (i) a buffer solution having a pH range of 3.5 to 4.5, (ii-a) a lyophilized bilirubin oxidase, (ii-b) a buffer solution for dissolving the lyophilized bilirubin oxidase, and (iii) a standard serum containing a prescribed amount of bilirubin, and a method for measurement of direct bilirubin by using the reagent. The reagent and method are useful for diagnosis of various diseases, for example, various hepatic diseases (e.g. jaundice) and cholepathia.
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