High sensitivity assay method
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United States of America Patent
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Dec 3, 1985
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N/A
app pub date -
Jun 22, 1983
filing date -
Jun 22, 1983
priority date (Note) -
Expired
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Abstract
A high sensitivity assay method of the coenzyme cyclic assay type, eliminates the need for a separate indication reaction, by using the cycling reaction itself as the indication reaction, according to the following steps: ##STR1## the thus-formed formazan is then colorimetrically measured, to give a highly sensitive determination of the quantity of the substance to be assayed, e.g. NAD.sup.+, NADP.sup.+, malic acid or .gamma.-aminobutyric acid in human serum.
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