Manufacture and use of fowl egg antibodies
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United States of America Patent
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Oct 29, 1985
Issued Date -
N/A
app pub date -
Jul 16, 1982
filing date -
Mar 22, 1978
priority date (Note) -
Expired
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Abstract
Immunological preparations are prepared by immunizing hens with an immunogen having a molecular or particle weight of at least 30,000, to a stage of hyper-immunization at which there occurs a plateau-like levelling-off of the antibody content of the serum. The immunogenicity of the immunogen can be enhanced by enlarging the immunogen particle mass. The eggs of the immunized hens are collected, the yolk is separated from the eggs, followed by separation of the lipid content of the yolk. The antibodies in the egg yolk are then rendered indispersable with the aid of a water-soluble linear filamentary non-charged polymer precipitant such as PEG and the indispersable antibodies are recovered. This precipitation of antibodies is advantageously preceded by a precipitation of caseinaceous proteins, lipid and yolk particles at lower polymer concentrations. Selected antibodies (IgY or IgG) can be concentrated by pH-controlled fractional precipitation with PEG. The immunological preparations are useful for micro-assays and in appropriate cases also for the treatment or prophylaxis of pathological conditions.
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