Liquid proteinase concentrate and method for preparation
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United States of America Patent
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Sep 24, 1985
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N/A
app pub date -
Jun 5, 1984
filing date -
Jun 5, 1984
priority date (Note) -
Expired
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Abstract
A storage stable liquid enzyme concentrate of Subtilisin Carlsberg containing 0.5-6.5 Anson Units of proteinase per gram of concentrate and method for preparing same. Solid form proteinase is extracted with 70-100 parts by volume propylene glycol, 30-0 parts by volume of water, then adjusted as necessary to 60-85% by wt. of the glycol. Stabilizing agents in the concentrate are 0.1-1 mol/Kg of a member selected from the group consisting of Na, K, and Ca glutamates, and glycinates, and acetamide, and also a calcium ion content of 0.04-0.5% by wt., pH range is 5-8.
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