Alcohol oxidase from Pichia-type yeasts
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United States of America Patent
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Sep 10, 1985
Issued Date -
N/A
app pub date -
Apr 27, 1981
filing date -
Jun 5, 1979
priority date (Note) -
Expired
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Abstract
A new alcohol oxidase is isolated from Pichia-type microorganisms in soluble or crystalline form. The crystalline novel enzyme is isolated by preparing an aqueous fluid containing cells of a Pichia-type microorganism, homogenizing the fluid and separating solids therefrom to produce an alcohol oxidase solution, adjusting the solution to have an ionic strength in the range of 0.05 to 0.01 in ionic strength to form a recovery range solution thereby causing the crystalline alcohol oxidase to form. The new enzyme is used to determine alcohol concentrations in fluid samples in which conditions are compatible with the enzyme's activity.
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