Separation of interleukin-2 from phytohemagglutinin by dye matrix chromatography
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United States of America Patent
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Apr 2, 1985
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N/A
app pub date -
Dec 14, 1982
filing date -
Dec 12, 1981
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Expired
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Abstract
Crude interleukin-2 extract is subjected to group-selective dye-ligand absorption chromatography in one or more stages of purification with a matrix-gel medium consisting of a Blue A ligand or variant thereof or of a Green A ligand in a concentration of approximately 1.5 to 3.0 mg/ml of expanded matrix at a pH of approximately 6.8 to 8.5, a temperature of approximately 4.degree. to 40.degree. C., and a flowthrough rate of approximately 10 to 100 ml/h, employing an eluent. Either PHA-free or extremely pure interleukin-2 is obtained, depending on the overall number of purification stages.
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