Chromatographically purifying proteolytic procoagulant enzyme from animal tissue extract
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United States of America Patent
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Jul 24, 1984
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app pub date -
Jun 23, 1982
filing date -
Jun 23, 1982
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Expired
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Abstract
A chromatographic procedure for the purification of a proteolytic procoagulant enzyme from extracts of human and animal tumors. The extracts are sequentially contacted with a first benzamide affinity chromatographic resin, an agarose filtration gel, a second benzamide affinity chromatographic resin and a phenyl-sepharose hydrophobic chromatographic resin. The resulting enzyme is capable of producing anti-procoagulant antibody which, which used in an immunoassay, is diagnostic for malignancy.
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